Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Frontiers in Microbiology

Article Title: Testosterone increases the virulence traits of uropathogenic Escherichia coli

doi: 10.3389/fmicb.2024.1422747

Figure Lengend Snippet: CFT073 colonization ( A,C 4 h) or invasion ( B , 2 h) of bladder epithelial cells after CFT073 pre-treated with or without testosterone (100 pg/mL, 2 ng/mL and 60 ng/mL). CFT073 (carrying a GFP-expressing plasmid) colonization was measured as mean fluorescence intensity (MFI) (A) and imaged (C) . Data are presented as mean ± SD of n = 3–8 independent experiments. The asterisk distinguishes statistical significance: * = p < 0.05, ** = p < 0.01, and *** = p < 0.001 vs. CFT073. Scale bar: 500 μm.

Article Snippet: Human bladder epithelial cell line 5,637 was purchased from American Type Culture Collection (Manassas, VA, United States) and cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1 mM non-essential amino acids (all from Thermo Fisher Scientific) at 37°C with 5% CO 2 .

Techniques: Expressing, Plasmid Preparation, Fluorescence

Journal: Frontiers in Microbiology

Article Title: Testosterone increases the virulence traits of uropathogenic Escherichia coli

doi: 10.3389/fmicb.2024.1422747

Figure Lengend Snippet: IL-1β (A) , IL-8 (B) and LDH (C) release from bladder epithelial cells stimulated with CFT073 MOI10 pre-treated with or without testosterone (100 pg/mL, 2 ng/mL and 60 ng/mL) at 4 h. Data are presented as mean ± SD of n = 6–8 independent experiments. The asterisks distinguish statistical significance: * = p < 0.05 and **** = p < 0.0001.

Article Snippet: Human bladder epithelial cell line 5,637 was purchased from American Type Culture Collection (Manassas, VA, United States) and cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1 mM non-essential amino acids (all from Thermo Fisher Scientific) at 37°C with 5% CO 2 .

Techniques:

Journal: Frontiers in Microbiology

Article Title: Testosterone increases the virulence traits of uropathogenic Escherichia coli

doi: 10.3389/fmicb.2024.1422747

Figure Lengend Snippet: RNASE7 (A) , LL37 (B) , beta-defensin 1 (C) and beta-defensin 2 (D) release from bladder epithelial cells stimulated with CFT073 MOI10 pre-treated with or without testosterone (100 pg/mL, 2 ng/mL and 60 ng/mL) at 4 h. Data are presented as mean ± SD of n = 3 independent experiments. The asterisks distinguish statistical significance: * = p < 0.05 and **** = p < 0.0001.

Article Snippet: Human bladder epithelial cell line 5,637 was purchased from American Type Culture Collection (Manassas, VA, United States) and cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1 mM non-essential amino acids (all from Thermo Fisher Scientific) at 37°C with 5% CO 2 .

Techniques:

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: SSRI treatment or 5-HT exposure marginally impact cell proliferation of breast cancer cell lines. Bar graphs depict relative proliferation assessed by Fluoroskan of MCF-10A ( a ), MCF-7 ( b ), MDA-MB-231 ( c ), MDA-MSC-hyb1 ( d ) and MDA- MSC-hyb3 ( e ) cells in response to treatment with 10 nM, 100 nM or 1000 nM fluoxetine (Fluo; yellow), sertraline (Sert; red), citalopram (Cita; blue) or 5-HT (grey) for indicated time periods normalized to DMSO-treated control cells (Ctrl; white). Data are depicted as mean ± SEM and summarize n = 9–10 (Ctrl) and at least n = 3 (SSRI/5-HT) experiments. P -values were determined by two-way ANOVA followed by Dunnett’s multiple comparison test; *** P < 0.001; ** P < 0.01; * P < 0.05 versus corresponding Ctrl.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: Effect of low-dose, long-term SSRI treatment or 5-HT exposure on breast cancer cell lines measured by MTT assay. Bar graphs depict relative absorbance of MTT assay of MCF-10A ( a ), MCF-7 ( b ), MDA-MB-231 ( c ), MDA-MSC-hyb1 ( d ) and MDA-MSC-hyb3 ( e ) cells in response to treatment with 100 nM or 1000 nM fluoxetine (Fluo; yellow), sertraline (Sert; red), citalopram (Cita; blue) or 5-HT (grey) for indicated time periods (96 h to 144 h) compared to DMSO-treated control cells (Ctrl; white) and cells receiving carboplatin (CP; 1000 nM; black). Data are depicted as mean ± SEM and summarize n = 3 experiments. P -values were determined by two-way ANOVA followed by Dunnett’s multiple comparison test; *** P < 0.001; ** P < 0.01; * P < 0.05 versus corresponding Ctrl.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques: MTT Assay

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: Fluoxetine and sertraline marginally increase glucose uptake in SK-OV-3 cells. Bar graphs depict relative cellular glucose uptake assessed by 18F-FDG incorporation of MCF-10A ( a ), MCF-7 ( b ) and MDA-MB-231 ( c ) breast cancer as well as SK-OV-3 ( d ) NIH:OVCAR-3 ( e ) and SK-MSC-hyb1 ( f ) ovarian cancer cells in response to stimulation with 1 µM fluoxetine (Fluo), sertraline (Sert), citalopram (Cita), or 5-HT relative to DMSO-treated control cells (Ctrl) for 72 h. Data, derived from n = 3 (b and e), n = 4 (a and c) or n = 5 (d and e) independent cell passages performed in duplicates, are depicted as means ± SEM. P -values were computed by one-way ANOVA followed by Dunnett’s multiple comparison test; ** P < 0.01.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques: Derivative Assay